解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Upon glucose restriction, eukaryotic cells upregulate oxidative metabolism to maintain homeostasis. Using genetic screens, we find that the mitochondrial serine hydroxymethyltransferase (SHMT2) is required for robust mitochondrial oxygen consumption and low glucose proliferation. SHMT2 catalyzes the first step in mito...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.01.024
更新日期:2018-02-15 00:00:00
abstract::Plant pattern recognition receptors (PRRs) perceive microbial and endogenous molecular patterns to activate immune signaling. The cytoplasmic kinase BIK1 acts downstream of multiple PRRs as a rate-limiting component, whose phosphorylation and accumulation are central to immune signal propagation. Previous work identif...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.12.026
更新日期:2018-02-01 00:00:00
abstract::RNA binding proteins (RBPs) regulate all aspects in the life cycle of RNA molecules. To elucidate the elements that guide RNA specificity, regulatory mechanisms, and functions of RBPs, methods that identify direct endogenous protein-RNA interactions are particularly valuable. UV crosslinking and immunoprecipitation (C...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2018.01.005
更新日期:2018-02-01 00:00:00
abstract::Defective ER-resident membrane proteins need to be ejected into the cytoplasm in order to be degraded by the proteasome, but the exact mechanism remains unclear. In this issue of Molecular Cell, Neal et al. (2018) reveal that the rhomboid pseudoprotease Dfm1 defines the central ERAD component for membrane protein disl...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2017.12.031
更新日期:2018-01-18 00:00:00
abstract::We have developed a highly parallel strategy, systematic gene-to-phenotype arrays (SGPAs), to comprehensively map the genetic landscape driving molecular phenotypes of interest. By this approach, a complete yeast genetic mutant array is crossed with fluorescent reporters and imaged on membranes at high density and con...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.12.016
更新日期:2018-01-18 00:00:00
abstract::N6-methyladenosine (m6A) and adenosine-to-inosine (A-to-I) editing are two of the most abundant RNA modifications, both at adenosines. Yet, the interaction of these two types of adenosine modifications is largely unknown. Here we show a global A-to-I difference between m6A-positive and m6A-negative RNA populations. Bo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.12.006
更新日期:2018-01-04 00:00:00
abstract::As sessile organisms, plants must adapt to variations in the environment. Environmental stress triggers various responses, including growth inhibition, mediated by the plant hormone abscisic acid (ABA). The mechanisms that integrate stress responses with growth are poorly understood. Here, we discovered that the Targe...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.12.002
更新日期:2018-01-04 00:00:00
abstract::The recent paper by Stadlmann et al. (2017) provides a novel algorithm for glycoproteomics in which complex glycopeptides can be identified in complex mixtures to aid in characterizing both the site of glycosylation and the glycan structure. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2017.11.024
更新日期:2017-12-07 00:00:00
abstract::R-loop, a three-stranded RNA/DNA structure, has been linked to induced genome instability and regulated gene expression. To enable precision analysis of R-loops in vivo, we develop an RNase-H-based approach; this reveals predominant R-loop formation near gene promoters with strong G/C skew and propensity to form G-qua...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.10.008
更新日期:2017-11-16 00:00:00
abstract::DNA lesions caused by UV damage are thought to be repaired solely by the nucleotide excision repair (NER) pathway in human cells. Patients carrying mutations within genes functioning in this pathway display a range of pathologies, including an increased susceptibility to cancer, premature aging, and neurological defec...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.10.021
更新日期:2017-11-16 00:00:00
abstract::Stress granules are mRNA-protein assemblies formed from nontranslating mRNAs. Stress granules are important in the stress response and may contribute to some degenerative diseases. Here, we describe the stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysis of purified stre...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.10.015
更新日期:2017-11-16 00:00:00
abstract::Microsatellite expansion diseases are caused by unstable tandem repeats of 3-10 nucleotides that become pathogenic beyond a threshold number of copies. Two groups present different approaches to reduce pathogenesis by targeting deactivated Cas9 to either the DNA (Pinto et al., 2017) or the RNA (Batra et al., 2017) rep...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2017.10.023
更新日期:2017-11-02 00:00:00
abstract::RING and U-box E3 ubiquitin ligases regulate diverse eukaryotic processes and have been implicated in numerous diseases, but targeting these enzymes remains a major challenge. We report the development of three ubiquitin variants (UbVs), each binding selectively to the RING or U-box domain of a distinct E3 ligase: mon...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.09.027
更新日期:2017-10-19 00:00:00
abstract::Autophagy is required for benign hepatic tumors to progress into malignant hepatocellular carcinoma. However, the mechanism is unclear. Here, we report that mitophagy, the selective removal of mitochondria by autophagy, positively regulates hepatic cancer stem cells (CSCs) by suppressing the tumor suppressor p53. When...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.09.022
更新日期:2017-10-19 00:00:00
abstract::Double-strand breaks (DSBs) of DNA in eukaryotic cells are predominantly repaired by non-homologous end joining (NHEJ). The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.08.021
更新日期:2017-10-05 00:00:00
abstract::Using improved techniques, Baptista et al. (2017) and Warfield et al. (2017) revisit fundamental questions about SAGA and TFIID function in yeast. They conclude that each complex independently contributes to the expression of all genes transcribed by RNA polymerase II. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.09.028
更新日期:2017-10-05 00:00:00
abstract::The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas nuclease system is a powerful tool for genome editing, and its simple programmability has enabled high-throughput genetic and epigenetic studies. These high-throughput approaches offer investigators a toolkit for functional interrogation of not...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2017.09.017
更新日期:2017-10-05 00:00:00
abstract::Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly d...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.08.014
更新日期:2017-10-05 00:00:00
abstract::Poly-ubiquitin chains direct protein substrates to the 26S proteasome, where they are removed by the deubiquitinase Rpn11 during ATP-dependent substrate degradation. Rapid deubiquitination is required for efficient degradation but must be restricted to committed substrates that are engaged with the ATPase motor to pre...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.07.023
更新日期:2017-09-07 00:00:00
abstract::Transcription is a source of genetic instability that can notably result from the formation of genotoxic DNA:RNA hybrids, or R-loops, between the nascent mRNA and its template. Here we report an unexpected function for introns in counteracting R-loop accumulation in eukaryotic genomes. Deletion of endogenous introns i...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.07.002
更新日期:2017-08-17 00:00:00
abstract::DNA methylation is an essential epigenetic mark in mammals that has to be re-established after each round of DNA replication. The protein UHRF1 is essential for this process; it has been proposed that the protein targets newly replicated DNA by cooperatively binding hemi-methylated DNA and H3K9me2/3, but this model le...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.07.012
更新日期:2017-08-17 00:00:00
abstract::Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engageme...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.06.010
更新日期:2017-07-20 00:00:00
abstract::Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.05.026
更新日期:2017-07-20 00:00:00
abstract::Alternative splicing (AS) generates isoform diversity for cellular identity and homeostasis in multicellular life. Although AS variation has been observed among single cells, little is known about the biological or evolutionary significance of such variation. We developed Expedition, a computational framework consisti...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.06.003
更新日期:2017-07-06 00:00:00
abstract::Infections can cause a multitude of stresses on the host and microbe. To detect potential infections, the mammalian immune system utilizes several families of pattern recognition receptors, which survey the intracellular and extracellular environments for microbial products. Members of each receptor family induce anti...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2017.05.009
更新日期:2017-06-15 00:00:00
abstract::The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated br...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.05.012
更新日期:2017-06-01 00:00:00
abstract::The protein 53BP1 plays a central regulatory role in DNA double-strand break repair. 53BP1 relocates to chromatin by recognizing RNF168-mediated mono-ubiquitylation of histone H2A Lys15 in the nucleosome core particle dimethylated at histone H4 Lys20 (NCP-ubme). 53BP1 relocation is terminated by ubiquitin ligases RNF1...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.04.009
更新日期:2017-05-18 00:00:00
abstract::Ubiquitin modification of proteins plays pivotal roles in the cellular response to DNA damage. Given the complexity of ubiquitin conjugation due to the formation of poly-conjugates of different linkages, functional roles of linkage-specific ubiquitin modification at DNA damage sites are largely unclear. We identify th...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.04.013
更新日期:2017-05-18 00:00:00
abstract::The HECT E3 ligases ubiquitinate numerous transcription factors and signaling molecules, and their activity must be tightly controlled to prevent cancer, immune disorders, and other diseases. In this study, we have found unexpectedly that peptide linkers tethering WW domains in several HECT family members are key regu...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.03.020
更新日期:2017-05-04 00:00:00
abstract::Cells exposed to hypoxia experience replication stress but do not accumulate DNA damage, suggesting sustained DNA replication. Ribonucleotide reductase (RNR) is the only enzyme capable of de novo synthesis of deoxyribonucleotide triphosphates (dNTPs). However, oxygen is an essential cofactor for mammalian RNR (RRM1/RR...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.03.005
更新日期:2017-04-20 00:00:00
abstract::The CRISPR-associated protein Cas12a (Cpf1), which has been repurposed for genome editing, possesses two distinct nuclease activities: endoribonuclease activity for processing its own guide RNAs and RNA-guided DNase activity for target DNA cleavage. To elucidate the molecular basis of both activities, we determined cr...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.03.016
更新日期:2017-04-20 00:00:00
abstract::Circular RNAs (circRNAs) are abundant and evolutionarily conserved RNAs of largely unknown function. Here, we show that a subset of circRNAs is translated in vivo. By performing ribosome footprinting from fly heads, we demonstrate that a group of circRNAs is associated with translating ribosomes. Many of these ribo-ci...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.02.021
更新日期:2017-04-06 00:00:00
abstract::In this issue of Molecular Cell, Taneja et al. (2017) uncover a dual role for the conserved chromatin remodeler Fft3 in the maintenance of silent heterochromatin and the suppression of replication barriers at euchromatic loci through controlled histone turnover. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.03.015
更新日期:2017-04-06 00:00:00
abstract::Dong et al. (2017) establish how the mitochondrial Ca2+ uniporter (MCU) integrates Ca2+ and oxidative stress signals by identifying a cysteine residue that controls MCU channel activity, a mechanism causing mitochondrial Ca2+ overload and cell death during oxidative stress. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.02.029
更新日期:2017-03-16 00:00:00
abstract::R loop, a transcription intermediate containing RNA:DNA hybrids and displaced single-stranded DNA (ssDNA), has emerged as a major source of genomic instability. RNaseH1, which cleaves the RNA in RNA:DNA hybrids, plays an important role in R loop suppression. Here we show that replication protein A (RPA), an ssDNA-bind...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.01.029
更新日期:2017-03-02 00:00:00
abstract::In budding yeast, the nuclear RNA surveillance system is active on all pre-mRNA transcripts and modulated by nutrient availability. To test the role of nuclear surveillance in reprogramming gene expression, we identified transcriptome-wide binding sites for RNA polymerase II and the exosome cofactors Mtr4 (TRAMP compl...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.01.005
更新日期:2017-03-02 00:00:00
abstract::Protein function originates from a cooperation of structural rigidity, dynamics at different timescales, and allostery. However, how these three pillars of protein function are integrated is still only poorly understood. Here we show how these pillars are connected in Protein Tyrosine Phosphatase 1B (PTP1B), a drug ta...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.01.014
更新日期:2017-02-16 00:00:00
abstract::Environmental cues provoke rapid transitions in gene expression to support growth and cellular plasticity through incompletely understood mechanisms. Lin28 RNA-binding proteins have evolutionarily conserved roles in post-transcriptional coordination of pro-growth gene expression, but signaling pathways allowing trophi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.12.025
更新日期:2017-02-02 00:00:00
abstract::Polycomb proteins are well-known epigenetic repressors with unexplained roles in chromatin folding. In this issue of Molecular Cell, Kundu et al. (2017) investigate the structures of PRC1-mediated domains in stem cells and probe their changes upon differentiation and in PRC knockouts. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.01.028
更新日期:2017-02-02 00:00:00
abstract::In this issue of Molecular Cell, Chereji et al. (2017) present new data on MNase-sensitive particles previously identified upstream of transcription start sites at many promoters in budding yeast, and they argue, based upon negative histone-ChIP results, that they are non-nucleosomal signals generated by transcription...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.01.010
更新日期:2017-02-02 00:00:00